p erk (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc p erk
P Erk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 13438 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+erk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+XP+Rabbit+mAb/pmc13618761-375-15-16
Average 99 stars, based on 13438 article reviews

p erk - by Bioz Stars, 2026-10

99/100 stars

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Article Title: Repurposing T-type calcium channel blocker Lomerizine as a therapeutic strategy for glioblastoma.
Article Snippet: The following primary antibodies were 464 used in this study: anti-Akt (Cell Signaling; #9272), anti-pAkt (Cell Signaling; #4058), anti-β-465 actin (FUJIFILM Wako Pure Chemical Corporation; 010-27841), anti-CDK4 (Cell Signaling; 466 #12790), anti-CDK6 (Cell Signaling; #13331), anti-ERK (Cell Signaling; #4695), anti-pERK 467 (Cell Signaling; #4370), anti-SOX2 (Gene Tex; GTX101507), anti-STAT3 (Cell Signaling; 468 #12640), anti-pSTAT3 S727 (Cell Signaling; #9134), anti-pSTAT3 Y705 (Cell Signaling; #9145), 469 and anti-PARP (Cell Signaling; #9542) , anti-Cleaved PARP (Cell Signaling; #5625).

Article Title: Novel Osteoblastogenic Activity of Magnolia kobus : The Pharmacological Potential for Osteoporosis
Article Snippet: The following agents were obtained from commercial sources: anti-DLX5 (sc-398150), anti-cathepsin K (sc-48353), and anti-osterix (sc-393325) (Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-RUNX2 (12556), anti-NFAT2 (NFATc1, 8032), anti-c-Fos (2250), anti-phospho-ERK (T202/Y204) (9101), anti-phospho-p38 MAPK (T180/Y182) (9215), anti-phospho-JNK (T183/Y185) (9251), anti-ERK (9102), anti-p38 MAPK (9212), anti-JNK (9252), anti-phospho-IKKα/β (2697), anti-IKKβ (8943), anti-phospho-IκBα (2859), anti-IκBα (4814), anti-actin (4970) antibodies, and rabbit and mouse IgG-horseradish peroxidase conjugates (Cell Signaling Technology, Beverly, MA, USA); and PD98059 (S1177) and SB203580 (S1076) (Selleck Chemicals, Houston, TX, USA).

Article Title: Salidroside Selectively Binds to SEC23A and Ameliorates Psychological Stress-Induced Hyperpigmentation.
Article Snippet: Primary antibodies were anti-Actin (Abcam, ab7817, Cambridge, UK); anti-MITF-M (Abcam, ab20663); anti-TYR (Abcam, ab180753); anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Abcam, ab8245); anti-p-CREB (Cell Signaling Technology, 9198); anti-CREB (Cell Signaling Technology, 9197, Massachusetts, MA, USA); anti-β-catenin (Cell Signaling Technology, 9567); anti-GSK (Cell Signaling Technology, 9562); anti-p-GSK (Cell Signaling Technology, 79774); anti-AKT (Cell Signaling Technology, 9272); anti-p-AKT (Cell Signaling Technology, 4060), anti-p-p38 (Cell Signaling Technology, 9211); anti-p38MAPK (Cell Signaling Technology, 9212); anti-p-ERK (Cell Signaling Technology, 9101); anti-ERK (Cell Signaling Technology, 9102); anti-p-JNK (Cell Signaling Technology, 9251); anti-JNK (Cell Signaling Technology, 9252); anti-MC1R (Cell Signaling Technology, 9595); anti-PKA (Abcam, ab32514); anti-NK1R (Abcam, ab75516); anti-tyrosinase (TYR)-related protein-1 (TRP-1; Abcam, ab235447); and anti-dopachrome tautomerase (DCT; Abcam, ab221144); anti-SEC23A (abclonal, A8613, Wuhan, Hubei, China).

Article Title: Salidroside Selectively Binds to SEC23A and Ameliorates Psychological Stress-Induced Hyperpigmentation
Article Snippet: Primary antibodies were anti-Actin (Abcam, ab7817, Cambridge, UK); anti-MITF-M (Abcam, ab20663); anti-TYR (Abcam, ab180753); anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; Abcam, ab8245); anti-p-CREB (Cell Signaling Technology, 9198); anti-CREB (Cell Signaling Technology, 9197, Massachusetts, MA, USA); anti-β-catenin (Cell Signaling Technology, 9567); anti-GSK (Cell Signaling Technology, 9562); anti-p-GSK (Cell Signaling Technology, 79774); anti-AKT (Cell Signaling Technology, 9272); anti-p-AKT (Cell Signaling Technology, 4060), anti-p-p38 (Cell Signaling Technology, 9211); anti-p38MAPK (Cell Signaling Technology, 9212); anti-p-ERK (Cell Signaling Technology, 9101); anti-ERK (Cell Signaling Technology, 9102); anti-p-JNK (Cell Signaling Technology, 9251); anti-JNK (Cell Signaling Technology, 9252); anti-MC1R (Cell Signaling Technology, 9595); anti-PKA (Abcam, ab32514); anti-NK1R (Abcam, ab75516); anti-tyrosinase (TYR)-related protein-1 (TRP-1; Abcam, ab235447); and anti-dopachrome tautomerase (DCT; Abcam, ab221144); anti-SEC23A (abclonal, A8613, Wuhan, Hubei, China).

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Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production
Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), anti-RUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543-1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

Article Title: Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production.
Article Snippet: Protein bands were visualized using the Amersham Imager 600 (GE) and quantified using ImageJ. .. Western blotting (WB) was performed with the following antibodies: anti-GAPDH (1:10,000, 60004-1-IG; Proteintech), anti-β-actin (1:10,000, 66009-1-IG; Proteintech), anti-cathepsin K (CTSK) (1:100, sc-48353; Santa Cruz), anti-NFATC1 (1:100, sc-7294; Santa Cruz), anti-matrix metallopeptidase 9 (MMP9) (1:1,000, 10375-2-AP; Proteintech), anti-RRM2 (1:100, sc-81850; Santa Cruz), anti-ITGB8 (1:1,000, 29775-1-AP; Proteintech), anti-OMD (1:1,000, ab154249; Abcam), antiRUNX2 (1:1,000, 20700-1-AP; Proteintech), anti-collagen type I (1:1,000, 14695-1-AP; Proteintech), anti-ATP Synthase F1 Subunit Alpha (ATP5A1) (1:10,000, 14676-1-AP; Proteintech), anti-ubiquinol-cytochrome c reductase core protein 2 (UQCRC2) (1:10,000, 67547-1-Ig; Proteintech), anti-succinate dehydrogenase subunits B (SDHB) (1:10,000, 10620-1-AP; Proteintech), anti-cytochrome oxidase subunit IV (MTCO4) (1:1,000, 11242-1-AP; Proteintech), anti-NADH-ubiquinone oxidoreductase ASHI subunit (NDUFB8) (1:10,000, 14794-1-AP; Proteintech), anti-FLAG (1:10,000, 20543- 1-AP; Proteintech), anti-HA (1:10,000, 51064-2-AP; Proteintech), anti-YAP (1:1,000, ET1608-30; HUABIO), anti-p-YAP (S127) (1:1,000, ET1611–69; HUABIO), anti-Smad2 (1:1,000, ET1604-22; HUABIO), anti-p-Smad2 (1:5,000, ET1612-32; HUABIO), anti-Smad3 (1:5,000, ET1607-41; HUABIO), anti-p-Smad3 (1:2,000, ET1609-41; HUABIO), anti-AKT (1:1,000, #9272, Cell Signaling Technology), anti-p-AKT (1:2,000, #4060, Cell Signaling Technology), anti-ERK (1:1,000, #9102, Cell Signaling Technology), anti-p-ERK (1:1,000, #4695, Cell Signaling Technology), anti-p38 (1:1,000, #9212, Cell Signaling Technology), anti-p-p38 (1:1,000, #4511, Cell Signaling Technology), anti-JNK (1:5,000, 51153-1-AP; Proteintech) and anti-p-JNK (1:1,000, 80024-1-RR; Proteintech). ..

Incubation:

Article Title: Synergistic Anticancer Effects of Apatinib and PD-L1 Inhibition in Breast Cancer.
Article Snippet: Equal amounts of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, Massachusetts, U.S.A). .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and then incubated overnight at 4 °C with the following primary antibodies purchased from Cell Signaling Technology (CST): anti-ERK (CST-4695, 1:1000), anti-p-ERK (CST-4370, 1:1000), 1 3 6 Page 2 of 12 Journal of Mammary Gland Biology and Neoplasia (2026) 31:6 anti-NF-κB (CST-8242, 1:1000), and anti-SLUG (CST-9585, 1:1000). .. Rabbit anti-β-actin antibody (Bioss, bsm-52262R, 1:5000) was used as a loading control.

Article Title: Integrative Multiomic Classification Reveals Distinct Origins and Evolutionary Trajectories of Thymic Epithelial Tumors
Article Snippet: After determining protein concentration in the cell extract via the bicinchoninic acid method (Thermo Fisher Scientific), 20 μg of protein was separated using SDS-PAGE with a 4% to 20% TGX gel (Bio-Rad) and transferred to a polyvinylidene difluoride membrane. .. Membranes were blocked for 1 hour with 5% skim milk in TBS and then incubated with primary antibodies, including anti-Flag (Sigma-Aldrich, #F1804, RRID: AB_262044), anti-AKT (Cell Signaling Technology, #9272, RRID: AB_329827), anti–phospho-AKT (Ser473; Cell Signaling Technology, #4058, RRID: AB_331168), anti-ERK (Cell Signaling Technology, #4695, RRID: AB_390779), anti–phospho-ERK (Thr202/Tyr204; Cell Signaling Technology, #4370, RRID: AB_2315112), and anti–β-actin (Sigma-Aldrich, #A5441, RRID: AB_476744). .. The membranes were washed and incubated with horseradish peroxidase–conjugated secondary antibodies, followed by detection with enhanced chemiluminescence according to the manufacturer’s instructions (Pierce).

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